SHAPE-Seq of telomerase RNA Physcomitrium Patens - SRA

SRX21980357: SHAPE-Seq of telomerase RNA Physcomitrium Patens
1 ILLUMINA (NextSeq 500) run: 795,307 spots, 240.2M bases, 96.4Mb downloads

Design: 10 pmol of in vitro transcribed RNA was denatured at 95 C for 2 minutes, quickly cooled down on the ice, mixed with a folding buffer (333 mM HEPES, pH 8.0; 333 mM NaCl; 33 mM MgCl2) and folded at 37 C for 20 minutes. This RNA was used for the modification reactions with 100 mM 1M7 (Sigma Aldrich) (+), DMSO (-), and DC (denaturing control). Modified RNAs prepared in triplicates were purified with SPRI beads and used for reverse transcription with Superscript-II in the presence of MnCl2 (SHAPE-Seq).

Submitted by: Central European Institute of Technology, Masaryk University

Study: Single molecule analysis reveals functionally relevant isoforms of telomerase RNA in moss

PRJNA1023189 • SRP464376 • All experiments • All runs

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The goal of the study is to determine the secondary structure of telomerase RNA in P. patens in vivo (using DMS-Seq) or in vitro (using SHAPE-Seq). cDNA with induced mutations was generated with TGIRT-II or Superscript-II in the presence of MnCl2 (SHAPE-Seq). Samples for sequencing were prepared in biological triplicates.

Sample:

SAMN37642559 • SRS19055625 • All experiments • All runs

Organism: Physcomitrium patens

Library:

Name: T7_1M7_DC_3

Instrument: NextSeq 500

Strategy: OTHER

Source: TRANSCRIPTOMIC

Selection: RT-PCR

Layout: PAIRED

Runs: 1 run, 795,307 spots, 240.2M bases, 96.4Mb

Run	# of Spots	# of Bases	Size	Published
SRR26270850	795,307	240.2M	96.4Mb	2024-01-01

ID:: 29888637

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